Extracellular Vesicles Archives

graphic on storage conditions of extracellular vesicles (EVs)

Applications

Use of Fluorescence Nanoparticle Tracking Analysis as a tool for quality assessment of EVs

Correct and standardized storage conditions of extracellular vesicles (EVs) are essential prerequisites for the stability of clinical samples and the production of consistent EV batches for therapeutic use.
The loss of EV concentration due to improper storage conditions is associated with a reduced activity and physical integrity.
Check out our new app note on the importance of EV storage conditions and how fluorescence nanoparticle tracking analysis can be used as a quality assessment tool.

27. June 2024

EV-membrane-associated nucleic acids labeled with SYBR™ Gold.

Applications

Detection of nucleic acid cargo in EVs by using Fluorescence Nanoparticle Tracking Analysis

The use of fluorescence nanoparticle tracking analysis (F-NTA) enables the detection of membrane-associated nucleic acids from EVs.

14. May 2024

Applications

Colocalization-NTA of multi-stained platelet derived MSC-EV preparations

After successfully showing C-NTA of double-stained MSC-EVs in our previous study (https://bit.ly/43GG0Yk) and confirming
the results with image flow cytometry, this note describes C-NTA of multi-stained MSC-EVs using a PMX-430 QUATT NTA
system with four lasers.

15. May 2023

Applications

NTA goes colocalization:
Detection and measurement of CD9 and CD41 on
double-stained human platelet derived MSC-EV
preparations

In this report, we describe the quantification of colocalization ratios on double-stained MSC-derived EVs using fluorochrome conjugated antibodies against the
cell surface antigens CD9 and CD41, which also serve as EV marker proteins.

1. March 2023

Application

Phenotyping of EVs by Multi-Wavelength Fluorescence Nanoparticle Tracking Analysis

F-NTA of fluorescent labelled bionanoparticles is an efficient technique for quantification of size and total concentration.

24. April 2022

Application

Evaluation of a Four Wavelength NTA Instrument for biomarker detection of cell line EVs

Shown with four-fold labeled EVs: Multi-wavelength NTA reduces total measurement time, minimizes the amount of sample required, and improves experimental reproducibility.

24. April 2022

Examination of an extracellular vesicle preparation from the colon cancer cell line HTC116. Comparison between the total particle count (grey; 100%), CD9 positives (violet; 3%), CD63 positives (green; 19%), CD81 positives (blue; 21%) and the biological vesicle count (red; 93%)

Application

Identification of Exosomes using the tetraspanins CD9, CD63, CD81 and Particle Metrix ZetaView® fluorescent Nanoparticle Tracking (F-NTA)

Nanoparticle Tracking Analysis (NTA) has emerged as the state-of the-art method for size and concentration characterization of exosomes and extracellular vesicles.

28. April 2020

Identification problem of particles in a classical scatter NTA video derived from an EV preparation

Extracellular Vesicles

Characterization of Extracellular Vesicles via fluorescence Nanoparticle Tracking Analysis (F-NTA)

Here we report a unique technology for rapid determination of membranous particles along with an analysis of multiple EV biomarkers. In spite of the experimental sophistication, the operational costs of our instrument are kept to a minimum.

26. August 2019

Comparison between the total particle concentration (gray) and the biological vesicle concentration (red).

Application

Purity check of Extracellular Vesicle preparations by Particle Metrix ZetaView® Nanoparticle Tracking Analysis

Over the past decade Nanoparticle Tracking Analysis (NTA) has emerged as an essential characterization tool for Extracellular Vesicle and Exosome research.

23. August 2019

NTA size measurement by detection of scattered light after excitation with 488nm (red line) and fluorescence light after excitation with 640nm (red line) and 405nm (green line) of HTC116 cell line derived exosomes stained with DAPI.

Application

Identification of DNA / RNA containing Extracellular Vesicles with DAPI and Particle Metrix ZetaView® Nanoparticle Tracking

Here we report an easy and quick method to analyse size and concentration of nucleic acid containing EVs by fluorescent NTA (f-NTA) after staining with DAPI.

22. August 2019

Figure 1: Particle concentration of total particles (100%) (grey) and CD41 positives derived from MSC (41,4%) and HTC116 (0%).

Application

Identification of platelet-derived Exosomes in plasma samples with Particle Metrix ZetaView® Nanoparticle Tracking

Here we report a unique method to identify platelet-derived Exosomes from plasma samples using specific fluorescently labelled CD41 antibody and the Particle Metrix ZetaView® instrument.

22. August 2019

A CellGS scientist uses the Particle Metrix ZetaView for the study of exosomes

Customer Interviews

Cell Guidance Systems use the ZetaView® from Particle Metrix for exosome research and quality control

Particle Metrix reports on how Cell Guidance Systems use the ZetaView® particle characterization system for exosome research and quality control.

12. July 2016

Michel Bremer from UK Essen using the Particle Metrix ZetaView system to characterise EVs.

Customer Interviews

The Institute for Transfusion Medicine at the University Hospital of Duisburg-Essen in Germany uses the ZetaView® from Particle Metrix to quantify extracellular vesicles

Particle Metrix reports on the work of the Giebel Working Group on the Developmental Biology of Stem Cells in the Institute for Transfusion Medicine at the University Hospital of Duisburg-Essen

7. June 2016

Jochen Dindorf with his Zetaview particle characterization system from Particle Metrix

Customer Interviews

The Translational Research Center at the University Hospital of Erlangen in Germany uses the ZetaView from Particle Metrix to quantify extracellular vesicles such as exosomes

Particle Metrix reports on the work of the Baur Laboratory in the Department of Dermatology at the University Hospital of Erlangen.

28. April 2016

Dr Yutao Liu from Augusta University with his Particle Metrix ZetaView® system

Biomarkers

Augusta University’s Department of Cellular Biology & Anatomy is studying exosomes using ZetaView® from Particle Metrix to aid in identification of potential biomarkers

Particle Metrix reports on the work in the Liu Laboratory at Augusta University which is studying exosomes where size and concentration are critical parameters.

31. March 2016

Technology

Applications

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Category: Extracellular Vesicles

Articles

Publications

A Mildly Acidic Environment Alters Pseudomonas aeruginosa Virulence and Causes Remodeling of the Bacterial Surface

A simple and available measurement of oncosEV dsDNA to protein ratio as a potential tumor marker

Annexin A5 Inhibits Endothelial Inflammation Induced by Lipopolysaccharide-Activated Platelets and Microvesicles via Phosphatidylserine Binding

Bacillus cereus extracellular vesicles act as shuttles for biologically active multicomponent enterotoxins

Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application

Biophysical and proteomic analyses of Pseudomonas syringae pv. tomato DC3000 extracellular vesicles suggest adaptive functions during plant infection

Bioprinted constructs that simulate nerve–bone crosstalk to improve microenvironment for bone repair

Bovine milk-derived extracellular vesicles enhance doxorubicin sensitivity in triple negative breast cancer cells by targeting metabolism and STAT signalling

Change in cytokine profiles released by mast cells mediated by lung cancer-derived exosome activation may contribute to cancer-associated coagulation disorders

Changes in urinary exosomal protein CALM1 may serve as an early noninvasive biomarker for diagnosing diabetic kidney disease

Characteristics of size-exclusion chromatography enriched porcine follicular fluid extracellular vesicles

Characterization of cannabis strain‑plant‑derived extracellular vesicles as potential biomarkers

Cord Blood Plasma and Placental Mesenchymal Stem Cells-Derived Exosomes Increase Ex Vivo Expansion of Human Cord Blood Hematopoietic Stem Cells While Maintaining Their Stemness

Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls

Different Biofluids, Small Extracellular Vesicles or Exosomes: Structural Analysis in Atherosclerotic Cardiovascular Disease Using Electron Microscopy Techniques

Effects of lysate/tissue storage at -80°C on subsequently extracted EVs of epithelial ovarian cancer tissue origins

Effects of Plasma-Derived Exosomal miRNA-19b-3p on Treg/T Helper 17 Cell Imbalance in Behçet’s Uveitis

Employing nanoparticle tracking analysis of salivary neuronal exosomes for early detection of neurodegenerative diseases

Endoplasmin Is a Hypoxia-Inducible Endoplasmic Reticulum-Derived Cargo of Extracellular Vesicles Released by Cardiac Cell Lines

Equine mesenchymal stem cell derived extracellular vesicle immunopathology biomarker discovery

Exosomal miRNA-223-3p derived from tumor associated macrophages promotes pulmonary metastasis of breast cancer 4T1 cells

Exosomes Derived from Dermatophagoides farinae Induce Allergic Airway Inflammation

Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung Injury

Extracellular vesicles derived from mouse adipose-derived mesenchymal stem cells promote diabetic corneal epithelial wound healing through NGF/TrkA pathway activation involving dendritic cells

Human adipose tissue‑derived small extracellular vesicles promote soft tissue repair through modulating M1‑to‑M2 polarization of macrophages

Hyperthermia promotes M1 polarization of macrophages via exosome‑mediated HSPB8 transfer in triple negative breast cancer

Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites

Improved Wound Healing and Skin Regeneration Ability of 3,2′-Dihydroxyflavone-Treated Mesenchymal Stem Cell-Derived Extracellular Vesicles

In situ formed scaffold with royal jelly-derived extracellular vesicles for wound healing

Independent human mesenchymal stromal cellderived extracellular vesicle preparations differentially attenuate symptoms in an advanced murine graft-versus-host disease model

M2 Macrophages-Derived Exosomal miRNA-23a-3p Promotes the Progression of Oral Squamous Cell Carcinoma by Targeting PTEN

Mesenchymal stromal cell exosomes enhance dental pulp cell functions and promote pulp-dentin regeneration

Novel neutrophil extracellular trap-related mechanisms in diabetic wounds inspire a promising treatment strategy with hypoxia-challenged small extracellular vesicles

Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos

Phosphatidylserine-positive extracellular vesicles boost effector CD8+ T cell responses during viral infection

Plasma-derived extracellular vesicles released after endurance exercise exert cardioprotective activity through the activation of antioxidant pathways

Plasma‑derived small extracellular vesicles unleash the angiogenic potential in head and neck cancer patients

Protein Biomarker Discovery Studies on Urinary sEV Fractions Separated with UF-SEC for the First Diagnosis and Detection of Recurrence in Bladder Cancer Patients

Quantitative imaging of vesicle-protein interactions reveals close cooperation among proteins

Regulation of Hsa-­miR-­4639-­5p expression and its potential role in the pathogenesis of Parkinson’s disease

SCARA5 in bone marrow stromal cell-derived exosomes inhibits colorectal cancer progression by inactivating the PI3K/Akt pathway

Selective pharmacological inhibition alters human carcinoma lung cell-derived extracellular vesicle formation

Sequencing and bioinformatics analysis of miRNA from rat endplate chondrogenic exosomes

SUR7 deletion in Candida albicans impacts extracellular vesicle features and delivery of virulence factors

Variability of extracellular vesicle release during storage of red blood cell concentrates is associated with differential membrane alterations, including loss of cholesterol-enriched domains

Adipose-derived mesenchymal stem cell-loaded β-chitin nanofiber hydrogel promote wound healing in rats

Blocking exosomal secretion aggravates 1,4-Benzoquinone-induced mitochondrial fission activated by the AMPK/MFF/Drp1 pathway in HL-60 cells

Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods

Exosomal lncRNA ATB Derived from Ovarian Cancer Cells Promotes Angiogenesis via Regulating miR-204-3p/TGFβR2 Axis

Exosomal miR-17-3p Alleviates Programmed Necrosis in Cardiac Ischemia/Reperfusion Injury by Regulating TIMP3 Expression

Extracellular Vesicles Derived from SIPA1high Breast Cancer Cells Enhance Macrophage Infiltration and Cancer Metastasis through Myosin-9

Fetal Membranes Contribute to Drug Transport across the Feto-Maternal Interface Utilizing the Breast Cancer Resistance Protein (BCRP)

Human Bone Marrow Stromal Cell Exosomes Ameliorate Periodontitis

Neutral Sphingomyelinase 2 Mediates Oxidative Stress Effects on Astrocyte Senescence and Synaptic Plasticity Transcripts

Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer

Profiling Blood Serum Extracellular Vesicles in Plaque Psoriasis and Psoriatic Arthritis Patients Reveals Potential Disease Biomarkers

Proteomic and Biochemical Analysis of Extracellular Vesicles Isolated from Blood Serum of Patients with Melanoma

Secretory Autophagosomes from Alveolar Macrophages Exacerbate Acute Respiratory Distress Syndrome by Releasing IL-1β

Serum-derived exosomes from SD rats induce inflammation in macrophages through the mTOR pathway

Small RNA cargo armed extracellular vesicles alleviate periodontitis in OVX mice via M2 macrophage polarization

Synergy of Human Platelet-Derived Extracellular Vesicles with Secretome Proteins Promotes Regenerative Functions

An engineered CD81-based combinatorial library for selecting recombinant binders to cell surface proteins: Laminin binding CD81 enhances cellular uptake of extracellular vesicles

Characterization of Extracellular Vesicles Labelled with a Lipophilic Dye Using Fluorescence Nanoparticle Tracking Analysis

Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus

Eribulin and Paclitaxel Differentially Alter Extracellular Vesicles and Their Cargo from Triple-Negative Breast Cancer Cells

Formation of a protein corona on the surface of extracellular vesicles in blood plasma

Hypoxia-stimulated ATM activation regulates autophagy-associated exosome release from cancer-associated fibroblasts to promote cancer cell invasion

MicroRNA Candidate Biomarkers for Parkinson’s Disease and Idiopathic REM Sleep Behavior Disorder

MicroRNAs are minor constituents of extracellular vesicles that are rarely delivered to target cells.

P2RX7 inhibition reduces breast cancer induced osteolytic lesions – implications for bone metastasis

Porphyromonas gingivalis Provokes Exosome Secretion and Paracrine Immune Senescence in Bystander Dendritic Cells

Regulation of Hsa-miR-4639-5p expression and its potential role in the pathogenesis of Parkinson’s disease