ZetaView® x30

Next Generation
Nanoparticle Tracking Analyzer

0x consumables
5x faster switching
10x faster cleaning
12x fluorescence channels
∞x statistics

…and much more!

ZetaView® x30

Next Generation NTA

0x consumables
5x faster switching
10x faster cleaning
12x fluorescence channels
∞x statistics

…and much more!

ZetaView® x30

Perfect for biological nanoparticles

Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses or virus-like particles are of growing interest in life sciences and nanomedicine. With the Nanoparticle Tracking Analysis technology, particles in a physiological buffer can be measured individually. You can watch what you measure in a video. With the ZetaView® x30 series, a major step has been taken towards convenience and speed for fluorescence studies of EV-antibody conjugates. ZetaView® combines the precision of new generation lasers for perfect illumination of NTA channels andvery short switching times with smart software – indispensable prerequisites for the challenging task of the quantification of biomarker colocalization scores.

Key Features & Benefits

Automated measurements are taken at 11 positions through the sample cell, providing a thorough and robust interrogation of samples, without need for additional accessories.
Greatly enhanced performance and less susceptibility to errors.
The optical setup is automatically optimized by the software, saving the user time preparing the instrument for use and removing subjective user input bias.
Analysis of over 2,000 particles in as little as 60 seconds.
Cleaning between samples requires only a quick flush. The new fixed cell design allows very quick access to the measuring cell with a bayonet lock.
Traffic-light system gives an instant indication of whether the sample is at a suitable concentration for measurement and indicates the dilution factor of the sample.
Measure size, concentration, zeta potential and fluorescence with one instrument. Compatible with the laboratory environment small footprint and data files

Fluorescence Nanoparticle Tracking Analysis (F-NTA)
measurements allow interrogation of subpopulations in the sample. A sensitive CMOS camera, up to 11 selective filters, and low bleaching performance yield high fluorescence sensitivity.

Just syringes and reference standards for scattering and fluorescence measurements (if applicable).
Size measurements do not need to be calibrated as the method is absolute.

Key Applications

Bio-nanoparticles:
  • Extracellular Vesicles (EVs)
  • Exosomes
  • Liposomes & Micelles
  • Protein Agglomerates
  • Viruses & Virus-Like-Particles (VLPs)
  • Drug Delivery
  • Fluorescent-labelled NPs
Low Concentration Samples:
  • Nanobubbles
  • Nanometals
  • Trace Particles
  • Quantum Dots
Particle Metrix ZetaView® x30 - Next Generation NTA

The x30 Series at a Glance

ZetaView® MONO
For the analysis of size, concentration, fluorescence, electrophoretic
mobility and sub-populations of individual nanoparticles
ZetaView® TWIN
For colocalization, size, concentration, zeta potential and cluster analysis
of individual particles in two different fluorescence channels
ZetaView® QUATT
For size, concentration, zeta potential and cluster analysis
of individual particles in four different fluorescence channels
Previous slide
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MONOTWINQUATT
Size✓✓✓
Concentration✓✓✓
Zeta potentialoptionaloptionaloptional
Fluorescenceoptional2 long pass filters4 long pass filters
Laser405 or
488 or
520 or
640 nm		405 & 520 nm or
405 & 640 nm or
488 & 640 nm or
520 & 640 nm		405 and
488 and
520 and
640 nm
Custom filter support*optional✓✓

For more information on the ZetaView® or to discuss your requirements, please contact us.

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Multi-Fluorescence NTA

Particle Metrix offers the versatility of the ZetaView® Nanoparticle Tracking Analysis on its well proven platform PMX-x30 keeping the instrument upgradable from one laser to four lasers. Changes between 405, 488, 520 or 640nm excitation lasers and between scattering and fluorescent modes are automated.

The movement and patterns of particles emitting scattered or fluorescent light are recorded in standard NTA or in Z-NTA surface charge electrophoresis mode. The new design of the PMX-x30 family with fixed cell assembly combines enhanced performance and reliable, error free measurements with simplicity and fast and easy instrument cleaning.

ZetaView® Colocalization NTA Feature (C-NTA)

Particle Metrix - Colocalization with ZetaView® QUATT

In the colocalization feature, measurements are performed in channel pairs enabling the user to phenotype fluorescently labelled nanoparticles at the next level. The colocalization feature is now available for QUATT systems offering flexibility of up to six excitation wavelength pair combinations and even more filter settings.

Concentration of fluorescently labelled bionanoparticles and colocalization scores can be determined from combinations of membrane dyes and/or fluorescently labelled antibodies. Read more about C-NTA and details about phenotyping of EVs and phages.

Applications

  • Phenotyping of extracellular vesicles (EVs)
  • Characterization of labelled viruses, phages and virus-like particles (VLPs)
  • Cargo determination of vesicles or micelles
  • Q-dots
  • Multi-fluorescent NP
  • and many more…
Read more about C-NTA

For more information on the ZetaView® or to discuss your requirements, please contact us.

Contact Us

Additional resources

Downloads

Applications

Videos & Webinars

Technologies

Publications

NTA Simulator

* Please contact us for further details

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