Phenotyping of EVs by Multi-Wavelength Fluorescence Nanoparticle Tracking Analysis

Multi-Wavelength F-NTA

Nanoparticle Tracking Analysis of fluorescent labelled bionanoparticles, such as vesicles or liposomes, is an efficient technique for quantification of size and total concentration. Analysis with single wavelength requires measurement of samples in sequence.

For the first time, we present an Nanoparticle Tracking Analysis instrument equipped with two lasers (TWIN-Laser NTA), allowing analysis of at least two different biomarkers on the same sample.
A Nanoparticle Tracking Analysis instrument with the combination of 405 and 488 nm was evaluated with fluorescent standards as well as antibody-labelled EVs.

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