Phenotyping of EVs by Multi-Wavelength Fluorescence Nanoparticle Tracking Analysis

Multi-Wavelength F-NTA

Nanoparticle Tracking Analysis of fluorescent labelled bionanoparticles, such as vesicles or liposomes, is an efficient technique for quantification of size and total concentration. Analysis with single wavelength requires measurement of samples in sequence.

For the first time, we present an Nanoparticle Tracking Analysis instrument equipped with two lasers (TWIN-Laser NTA), allowing analysis of at least two different biomarkers on the same sample.
A Nanoparticle Tracking Analysis instrument with the combination of 405 and 488 nm was evaluated with fluorescent standards as well as antibody-labelled EVs.

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The x30 Series at a Glance

ZetaView® TWIN
For colocalization, size, concentration, zeta potential and cluster analysis
of individual particles in two different fluorescence channels
ZetaView® MONO
For the analysis of size, concentration, fluorescence, electrophoretic
mobility and sub-populations of individual nanoparticles
ZetaView® QUATT
For size, concentration, zeta potential and cluster analysis
of individual particles in four different fluorescence channels
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