extracellular vesicles Archives

graphic on storage conditions of extracellular vesicles (EVs)

Applications

Use of Fluorescence Nanoparticle Tracking Analysis as a tool for quality assessment of EVs

Correct and standardized storage conditions of extracellular vesicles (EVs) are essential prerequisites for the stability of clinical samples and the production of consistent EV batches for therapeutic use.
The loss of EV concentration due to improper storage conditions is associated with a reduced activity and physical integrity.
Check out our new app note on the importance of EV storage conditions and how fluorescence nanoparticle tracking analysis can be used as a quality assessment tool.

27. June 2024

EV-membrane-associated nucleic acids labeled with SYBR™ Gold.

Applications

Detection of nucleic acid cargo in EVs by using Fluorescence Nanoparticle Tracking Analysis

The use of fluorescence nanoparticle tracking analysis (F-NTA) enables the detection of membrane-associated nucleic acids from EVs.

14. May 2024

Applications

Colocalization-NTA of multi-stained platelet derived MSC-EV preparations

After successfully showing C-NTA of double-stained MSC-EVs in our previous study (https://bit.ly/43GG0Yk) and confirming
the results with image flow cytometry, this note describes C-NTA of multi-stained MSC-EVs using a PMX-430 QUATT NTA
system with four lasers.

15. May 2023

Applications

NTA goes colocalization:
Detection and measurement of CD9 and CD41 on
double-stained human platelet derived MSC-EV
preparations

In this report, we describe the quantification of colocalization ratios on double-stained MSC-derived EVs using fluorochrome conjugated antibodies against the
cell surface antigens CD9 and CD41, which also serve as EV marker proteins.

1. March 2023

Technology

Nanoparticle Tracking Analysis

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Tag: extracellular vesicles

Articles

Use of Fluorescence Nanoparticle Tracking Analysis as a tool for quality assessment of EVs

Detection of nucleic acid cargo in EVs by using Fluorescence Nanoparticle Tracking Analysis

Colocalization-NTA of multi-stained platelet derived MSC-EV preparations

NTA goes colocalization:Detection and measurement of CD9 and CD41 ondouble-stained human platelet derived MSC-EVpreparations

Publications

Endothelial derived, secreted long non-coding RNAs Gadlor1 and Gadlor2 aggravate cardiac remodeling

Four-dimensional trapped ion mobility spectrometry proteomics reveals circulating extracellular vesicles encapsulated drivers of nasopharyngeal carcinoma distant dissemination

Improvement of androgenic alopecia by extracellular vesicles secreted from hyaluronic acid‑stimulated induced mesenchymal stem cells

Involvement of extracellular vesicle microRNA clusters in developing healthy and Rett syndrome brain organoids

Thermosensitive hydrogel as a sustained release carrier for mesenchymal stem cellderived extracellular vesicles in the treatment of intrauterine adhesion

Annexin A5 Inhibits Endothelial Inflammation Induced by Lipopolysaccharide-Activated Platelets and Microvesicles via Phosphatidylserine Binding

Application of Circulating Large Extracellular Vesicles as Biomarkers in Type 1 Diabetes Mellitus and Pregnancy

Bacillus cereus extracellular vesicles act as shuttles for biologically active multicomponent enterotoxins

Biophysical and proteomic analyses of Pseudomonas syringae pv. tomato DC3000 extracellular vesicles suggest adaptive functions during plant infection

Characteristics of size-exclusion chromatography enriched porcine follicular fluid extracellular vesicles

Depletion of abundant plasma proteins for extracellular vesicle proteome characterization: benefits and pitfalls

Effects of lysate/tissue storage at -80°C on subsequently extracted EVs of epithelial ovarian cancer tissue origins

Employing nanoparticle tracking analysis of salivary neuronal exosomes for early detection of neurodegenerative diseases

Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung Injury

Extracellular vesicle‑associated tyrosine kinase‑like orphan receptors ROR1 and ROR2 promote breast cancer progression

Identification and analysis of the DNA content of small extracellular vesicles isolated from Leishmania parasites

In situ formed scaffold with royal jelly-derived extracellular vesicles for wound healing

Independent human mesenchymal stromal cellderived extracellular vesicle preparations differentially attenuate symptoms in an advanced murine graft-versus-host disease model

Mesenchymal stromal cell exosomes enhance dental pulp cell functions and promote pulp-dentin regeneration

Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos

Phosphatidylserine-positive extracellular vesicles boost effector CD8+ T cell responses during viral infection

Plasma extracellular vesicle proteins as promising noninvasive biomarkers for diagnosis of idiopathic pulmonary fibrosis

Plasma-derived extracellular vesicles released after endurance exercise exert cardioprotective activity through the activation of antioxidant pathways

Protein Biomarker Discovery Studies on Urinary sEV Fractions Separated with UF-SEC for the First Diagnosis and Detection of Recurrence in Bladder Cancer Patients

Selective pharmacological inhibition alters human carcinoma lung cell-derived extracellular vesicle formation

Src family kinases engage differential pathways for encapsulation into extracellular vesicles

SUR7 deletion in Candida albicans impacts extracellular vesicle features and delivery of virulence factors

The Proteome of Circulating Large Extracellular Vesicles in Diabetes and Hypertension

Extracellular Vesicles Derived from SIPA1high Breast Cancer Cells Enhance Macrophage Infiltration and Cancer Metastasis through Myosin-9

Lipid-based strategies used to identify extracellular vesicles in flow cytometry can be confounded by lipoproteins: Evaluations of annexin V, lactadherin, and detergent lysis

Neutral Sphingomyelinase 2 Mediates Oxidative Stress Effects on Astrocyte Senescence and Synaptic Plasticity Transcripts

Proteomic and Biochemical Analysis of Extracellular Vesicles Isolated from Blood Serum of Patients with Melanoma

Eribulin and Paclitaxel Differentially Alter Extracellular Vesicles and Their Cargo from Triple-Negative Breast Cancer Cells

MicroRNAs are minor constituents of extracellular vesicles that are rarely delivered to target cells.

Proteomic analysis of extracellular vesicles from tick hemolymph and uptake of extracellular vesicles by salivary glands and ovary cells

BMSCs protect against renal ischemia-reperfusion injury by secreting exosomes loaded with miR-199a-5p that target BIP to inhibit endoplasmic reticulum stress at the very early reperfusion stages

Bronchial epithelial cell extracellular vesicles ameliorate epithelial–mesenchymal transition in COPD pathogenesis by alleviating M2 macrophage polarization

Detection of AML-specific mutations in pediatric patient plasma using extracellular vesicle–derived RNA

Extracellular vesicle‑delivered miR‑505‑5p, as a diagnostic biomarker of early lung adenocarcinoma, inhibits cell apoptosis by targeting TP53AIP1

Extracellular Vesicles and Ebola Virus: A New Mechanism of Immune Evasion

Extracellular vesicles derived from Malassezia furfur stimulate IL-6 production in keratinocytes as demonstrated in in vitro and in vivo models

Hypoxia preconditioned renal tubular epithelial cell-derived extracellular vesicles alleviate renal ischaemia-reperfusion injury mediated by the HIF-1α/Rab22 pathway and potentially affected by microRNAs

Individual Immune-Modulatory Capabilities of MSC-Derived Extracellular Vesicle (EV) Preparations and Recipient-Dependent Responsiveness

Innate extracellular vesicles from melanoma patients suppress β-catenin in tumor cells by miRNA-34a

Innovative Precipitation-Flocculation Process for Treating Turbid Waters from Gualaxo do Norte River, Brazil

Matrix stiffness regulates microvesicle-induced fibroblast activation

Antiretroviral Drugs Alter the Content of Extracellular Vesicles from HIV-1-Infected Cells

Beta cell extracellular vesicle miR-21-5p cargo is increased in response to inflammatory cytokines and serves as a biomarker of type 1 diabetes

Circular RNA expression in extracellular vesicles isolated from serum of patients with endometrial cancer

Communication of prostate cancer cells with bone cells via extracellular vesicle RNA; a potential mechanism of metastasis.

Detailed analysis of the plasma extracellular vesicle proteome after separation from lipoproteins

Differential fluorescence nanoparticle tracking analysis for enumeration of the extracellular vesicle content in mixed particulate solutions

Ebola Virus VP40 Modulates Cell Cycle and Biogenesis of Extracellular Vesicles

Effect of plasma-derived extracellular vesicles on erythrocyte deformability in polymicrobial sepsis

Exosomes from asbestos-exposed cells modulate geneexpression in mesothelial cells

Extracellular Vesicle-Associated mir-21 and mir-144 Are Markedly Elevated in Serum of Patients With Hepatocellular Carcinoma.

Extracellular vesicle-carried Jagged-1 inhibits HUVEC sprouting in a 3D microenvironment

Extracellular vesicle-mediated long-range communication in stressed retinal pigment epithelial cell monolayers

Extracellular vesicles or free circulating DNA: where to search for BRAF and cKIT mutations?

Fetal Bovine Serum-Derived Extracellular Vesicles Persist within Vesicle-Depleted Culture Media

Glucocerebrosidase deficiency promotes protein aggregation through dysregulation of extracellular vesicles

Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Modify Microglial Response and Improve Clinical Outcomes in Experimental Spinal Cord Injury

Integrated Nanoscale Deterministic Lateral Displacement Arrays for Separation of Extracellular Vesicles from Clinically-Relevant Volumes of Biological Samples

Lack of chitin synthase genes impacts capsular architecture and cellular physiology in Cryptococcus neoformans

Large-scale, cross-flow based isolation of highly pure and endocytosis-competent extracellular vesicles

Lipopolysaccharide-Preconditioned Periodontal Ligament Stem Cells Induce M1 Polarization of Macrophages through Extracellular Vesicles

NTA goes colocalization:
Detection and measurement of CD9 and CD41 on
double-stained human platelet derived MSC-EV
preparations