Category: Viruses

Your search for Viruses returned 2 articles and 12 publications

Articles

Example of an F-NTA measurement of a bacteriophage Phi6 preparation labelled with Sybr™Gold nucleic acid stain (blue curve) and Cellbrite® Fix 640 lipid layer stain (red curve) compared to scatter-based NTA (grey curve). Purity was calculated to be 82% for dsRNA and 85% for lipid layer containing phage particles.
Application

Rapid determination of purity, integrity and titer
of viruses by Fluorescence Nanoparticle Tracking
Analysis (F-NTA) using the bacteriophage Phi6

Determination of the titer of viruses and bacteriophages is an indispensable key technology in virological research and for diagnostic purposes. Depending on the method used, the measurements are either qualitative or quantitative, very time-consuming, and do not provide information about integrity or aggregation behaviour of the virus particles. Nanoparticle Tracking Analysis with the Particle Metrix ZetaView® instrument allows the user to perform a rapid concentration determination of virus particles. Using the bacteriophage Phi6 as an example, we show how titer, purity and integrity of phage particles can be measured quickly and reliably using the fluorescence detection capability of the Particle Metrix ZetaView® instrument.

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Publications

Salivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection

Carina Conzelmann, Rüdiger Groß, Min Zou, Franziska Krüger, André Görgens, Manuela O Gustafsson, Samir El Andaloussi, Jan Münch & Janis A. Müller

Journal of Extracellular Vesicles

2020

ApplicationsConcentration MeasurementExtracellular VesiclesSize MeasurementVesiclesViruses

Corona virusEV isolationEV purificationsaEVssalivary EVsSARS-CoV-2Zika virus

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Ebola Virus VP40 Modulates Cell Cycle and Biogenesis of Extracellular Vesicles

Michelle L Pleet, James Erickson, Catherine DeMarino, Robert A Barclay, Maria Cowen, Benjamin Lepene, Janie Liang, Jens H Kuhn, Laura Prugar, Spencer W Stonier, John M Dye, Weidong Zhou, Lance A Liotta, M Javad Aman, Fatah Kashanchi

The Journal of Infectuos Diseases

2018

ApplicationsConcentration MeasurementExtracellular VesiclesSize MeasurementTechnical NanoparticlesVesiclesViruses

Apoptosiscell cycleebola treatmentEbola virusEBOVEBOV pathogenesiEBOV treatmentEV biogenesisEVsextracellular vesiclesmonocyte apoptosispoptosis; cell cycleT cell apoptosisVP40

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