Category: Viruses

Your search for Viruses returned 2 articles and 12 publications

Articles

Example of an F-NTA measurement of a bacteriophage Phi6 preparation labelled with Sybr™Gold nucleic acid stain (blue curve) and Cellbrite® Fix 640 lipid layer stain (red curve) compared to scatter-based NTA (grey curve). Purity was calculated to be 82% for dsRNA and 85% for lipid layer containing phage particles.
Application

Rapid determination of purity, integrity and titer
of viruses by Fluorescence Nanoparticle Tracking
Analysis (F-NTA) using the bacteriophage Phi6

Determination of the titer of viruses and bacteriophages is an indispensable key technology in virological research and for diagnostic purposes. Depending on the method used, the measurements are either qualitative or quantitative, very time-consuming, and do not provide information about integrity or aggregation behaviour of the virus particles. Nanoparticle Tracking Analysis with the Particle Metrix ZetaView® instrument allows the user to perform a rapid concentration determination of virus particles. Using the bacteriophage Phi6 as an example, we show how titer, purity and integrity of phage particles can be measured quickly and reliably using the fluorescence detection capability of the Particle Metrix ZetaView® instrument.

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18. July 2022

Publications

Hydrodynamic characterization of a vesicular stomatitis virus‑based oncolytic virus using analytical ultracentrifugation

Wawra, Simon; Kessler, Sophia; Egel, Arina; Solzin, Johannes; Burkert, Oliver; Hochdorfer, Daniel

European Biophysics Journal

2023

ApplicationsConcentration MeasurementSize MeasurementViruses

Analytical ultracentrifugationnanoparticle tracking analysisOncolytic virusVesicular stomatitis virus

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Single-Pass Tangential Flow Filtration (SPTFF) of Nanoparticles: Achieving Sustainable Operation with Dilute Colloidal Suspensions for Gene Therapy Applications

Chaubal, Akshay S.; Zydney, Andrew L.

Membranes 13 (4)

2023

ApplicationsIsolation TechniquesMicroparticlesSize MeasurementTechnical NanoparticlesViruses

concentration polarizationcontinuous processingcritical fluxgenegene therapylentivirusnanoparticlesSPTFFtherapyultrafiltrationviral vectors

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Development of surface engineered antigenic exosomes as vaccines for respiratory syncytial virus

Suyeon Hong, Shaobo Ruan, Zachary Greenberg, Mei He, and Jodi L. McGill

Scientific Reports

2021

ExosomesExtracellular VesiclesVaccinesVesiclesVirusesZeta Potential

dendritic cell exoxomesMHCRespiratory syncytial virus

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Salivary extracellular vesicles inhibit Zika virus but not SARS-CoV-2 infection

Carina Conzelmann, Rüdiger Groß, Min Zou, Franziska Krüger, André Görgens, Manuela O Gustafsson, Samir El Andaloussi, Jan Münch & Janis A. Müller

Journal of Extracellular Vesicles

2020

ApplicationsConcentration MeasurementExtracellular VesiclesSize MeasurementVesiclesViruses

Corona virusEV isolationEV purificationsaEVssalivary EVsSARS-CoV-2Zika virus

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Changes of microRNAs expression profiles from red swamp crayfish (Procambarus clarkia) hemolymph exosomes in response to WSSV infection

HuiYang, XixiLi, Jiaojun Ji, ChunyouYuan, Xiaojian Gao, Yingying Zhang, Cheng Lu, Fenggang Li, Xiaojun Zhang

Fish & Shellfish Immunology

2019

ApplicationsConcentration MeasurementExtracellular VesiclesSize MeasurementTechnical NanoparticlesVesiclesViruses

deep sequencingexosomeexosomesfishimmunologymicroRNAmiRNAP. clarkiaprocambarus clarkiashellfishWSSVWSSV virus

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Exosomes Released from Rabies Virus-Infected Cells May be Involved in the Infection Process

Jingyu Wang, Fan Wu. Chuntian Liu, Wenwen Dai, Yawei Teng, Weiheng Su, Wei Kong, Feng Gao et al.

Virologica Sinica

2019

ApplicationsConcentration MeasurementExtracellular VesiclesSize MeasurementTechnical NanoparticlesVesiclesViruses

exosome isolationexosomesrabiesrabies infectionrabies virus

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Antiretroviral Drugs Alter the Content of Extracellular Vesicles from HIV-1-Infected Cells

C DeMarino, ML Pleet, M Cowen, RA Barclay et al.

Scientific Reports

2018

ApplicationsConcentration MeasurementExtracellular VesiclesSize MeasurementVesiclesViruses

cART-altered EV contentCD63CD81CD9DemeclocyclineDoxycyclineextracellular vesiclesFDA-approved drugsHIV-1MethacyclineMinocyclinenon-coding viral RNAOxycyclineOxytetracyclineSCRT pathwayTAR RNtetracyclineVPS4

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Ebola Virus VP40 Modulates Cell Cycle and Biogenesis of Extracellular Vesicles

Michelle L Pleet, James Erickson, Catherine DeMarino, Robert A Barclay, Maria Cowen, Benjamin Lepene, Janie Liang, Jens H Kuhn, Laura Prugar, Spencer W Stonier, John M Dye, Weidong Zhou, Lance A Liotta, M Javad Aman, Fatah Kashanchi

The Journal of Infectuos Diseases

2018

ApplicationsConcentration MeasurementExtracellular VesiclesSize MeasurementTechnical NanoparticlesVesiclesViruses

Apoptosiscell cycleebola treatmentEbola virusEBOVEBOV pathogenesiEBOV treatmentEV biogenesisEVsextracellular vesiclesmonocyte apoptosispoptosis; cell cycleT cell apoptosisVP40

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Exosome markers associated with immune activation and oxidative stress in HIV patients on antiretroviral therapy

S Chettimada, DR Lorenz, V Misra, ST Dillon et al.

Scientific Reports

2018

ApplicationsConcentration MeasurementExtracellular VesiclesSize MeasurementVesiclesViruses

CD63CD81CD9CRPD14exosome cargoHIVHLA-AHLA-Bimmunological markersimmunomodulationoxidative stressplasma exosome

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Nef Secretion into Extracellular Vesicles or Exosomes Is Conserved across Human and Simian Immunodeficiency Viruses

Ryan P. McNamara, Lindsey M. Costantini, T. Alix Myers, Blake Schouest, Nicholas J. Maness, Jack D. Griffith, Blossom A. Damania, Andrew G. MacLean, Dirk P. Dittmer.

mBio

2018

ApplicationsConcentration MeasurementExosomesExtracellular VesiclesSize MeasurementVesiclesViruses

AIDSCD4CD63CD81exosomesextracellular vesiclesFlotillinHIVlentivirusesmicrovesiclesNefSIV

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Extracellular vesicles from KSHV-infected endothelial cells activate the complement system

JL Kang, J Park, SJ Gao, MS Lee.

Oncotarget

2017

ApplicationsConcentration MeasurementExtracellular VesiclesMicroparticlesSize MeasurementTechnical NanoparticlesVesiclesViruses

Alternative Complement PathwayCD63CD81complement systemendothelial cellsextracellular vesiclesherpesvirusHSP70innate immunityKSHVNF-κB pathwayproteomics analysis

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Single-Particle Discrimination of Retroviruses from Extracellular Vesicles by Nanoscale Flow Cytometry

Vera A. Tang, Tyler M. Renner, Anna K. Fritzsche, Dylan Burger & Marc-André Langlois.

Scientific Reports - Nature

2017

ApplicationsConcentration MeasurementExosomesExtracellular VesiclesSize MeasurementVesiclesVirusesZeta Potential

eGFPextracellular vesiclesHIVMoloney murine leukemia virusnanoscale flow cytometryRetrovirusSingle particle discriminationVirus-containing supernatant

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