
High Throughput ZetaView® System vs High Cost of Other Methods
Techology Note Introduction In addition to using Nano Particle Tracking Analysis (NTA) to measurement the size distribution and concentration of EV samples, both Microfluidic Resistive
0x consumables
5x faster switching
10x faster cleaning
12x fluorescence channels
∞x statistics
…and much more!
For size, concentration, zeta potential and cluster analysis of individual particles in four different fluorescence channels.
We measure your products in a measuring range of 0.8 nm to 300 μm, if possible in original concentration
Particle Metrix, developers of versatile particle characterization solutions for the life sciences, report on the work in the Liu Laboratory at Augusta University which is studying exosomes where size and concentration are critical parameters.
Dr Yutao Liu is an Associate Professor in the Department of Cellular Biology & Anatomy at Augusta University. The research goal of his group is to identify potential biomarkers for several human diseases. As a human genetics research lab, they are interested in studying how exosomes and its contents contribute to the development of glaucoma, such as primary open-angle glaucoma and exfoliation glaucoma. They use human aqueous tumor and serum samples to explore exosomes and their contents. They are further interested in studying the role of exosomes in serum and other body fluids in relation to aging, smoking cessation and other age-related disorders. The Laboratory collaborates with many researchers interested in the study of exosomes.
Dr Liu’s group started to study exosomes in 2012. In the early days, they learned that it was very critical to know the size and the concentration of nanoparticles in their study samples prior to the characterization of protein and RNA contents. He selected the ZetaView® system from Particle Metrix as it provided quick, efficient and reliable measurements of particle size and concentration. Dr Liu said “Its innovative design with 11-position measurement throughout the cell enables the high accuracy and reliability of the actual measurement by avoiding measuring the same particles repeatedly. In addition, ZetaView measures zeta potential with the same instrument. After comparing ZetaView with other available platforms, we are confident that ZetaView matches to our research needs better than other commercial platforms. Having tried other systems at the core facility at UNC, Chapel Hill, we purchased our ZetaView in 2014 and now use it exclusively.”
Describing why he thinks the design of the instrument is particularly strong, Dr Liu continues: “In comparing to other particle characterization systems, ZetaView is superb in providing more accurate measurement about particle size and concentration. The 11-position measurement together with the 10x lens enables the measurement of larger volume in the actual sample by avoiding measuring the same particles again and again. The software interface is very user-friendly and Particle Metrix has provided great technical support to solve any requests we had for change. The recent improvements in the hardware and software algorithms make particle characterization much easier, less time-consuming and more accurate. Also, ZetaView is able to measure zeta potential very reliably. We have done hundreds of measurements on zeta potential. So far, we have characterized particle size and concentration with over 1,000 samples.”
Techology Note Introduction In addition to using Nano Particle Tracking Analysis (NTA) to measurement the size distribution and concentration of EV samples, both Microfluidic Resistive
Along with generally accepted methods are some “tricks of the trade” such as the addition of Tween® or BSA; however, some of those additions are problematic.
Application Note Download Abstract Determination of the titer of viruses and bacteriophages is an indispensable key technology in virological research and for diagnostic purposes. Depending
Platinum Presenter Talk at ISEV 2022 in Lyon Dr. Clemens Helmbrecht NTA is a multi-parameter technology Nanoparticle Tracking Analysis (NTA) is well known for measuring
The preparation of high-value biological material, such as proteins, requires a multi-step procedure of separation and purification. Each step must be optimized to reach full efficiency of yield.
Making a great exosome research tool even better, SBI has developed ExoGlow TM -NTA, a proprietary dye that enables fluorescent analysis of only the extracellular vesicles (EVs) present in a heterogeneous sample.
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