Your search for F-NTA returned 14 articles, 0 publications and 4 pages
After successfully showing C-NTA of double-stained MSC-EVs in our previous study (https://bit.ly/43GG0Yk) and confirming
the results with image flow cytometry, this note describes C-NTA of multi-stained MSC-EVs using a PMX-430 QUATT NTA
system with four lasers.
In this new application note we describe a quick determination of the physical titer of coronaviruses by fluorescence-based Nanoparticle Tracking Analysis and specific spike antibody recognition.
In this report, we describe the quantification of colocalization ratios on double-stained MSC-derived EVs using fluorochrome conjugated antibodies against the
cell surface antigens CD9 and CD41, which also serve as EV marker proteins.
Determination of the titer of viruses and bacteriophages is an indispensable key technology in virological research and for diagnostic purposes. Depending on the method used, the measurements are either qualitative or quantitative, very time-consuming, and do not provide information about integrity or aggregation behaviour of the virus particles. Nanoparticle Tracking Analysis with the Particle Metrix ZetaView® instrument allows the user to perform a rapid concentration determination of virus particles. Using the bacteriophage Phi6 as an example, we show how titer, purity and integrity of phage particles can be measured quickly and reliably using the fluorescence detection capability of the Particle Metrix ZetaView® instrument.
Making a great exosome research tool even better, SBI has developed ExoGlow TM -NTA, a proprietary dye that enables fluorescent analysis of only the extracellular vesicles (EVs) present in a heterogeneous sample.
F-NTA of fluorescent labelled bionanoparticles is an efficient technique for quantification of size and total concentration.
Traditionally, detection of fluorescent particles (F-NTA) has been shown to be challenging due to bleaching effects known from laser induced fluorescence techniques (LIF).
From the same sample you get the parameters: number concentration, size, electrophoresis surface charge, sub-populations and fluorescence phenotyping in little time.
Nanoparticle Tracking Analysis (NTA) has emerged as the state-of the-art method for size and concentration characterization of exosomes and extracellular vesicles.
Here we report a unique technology for rapid determination of membranous particles along with an analysis of multiple EV biomarkers. In spite of the experimental sophistication, the operational costs of our instrument are kept to a minimum.
Over the past decade Nanoparticle Tracking Analysis (NTA) has emerged as an essential characterization tool for Extracellular Vesicle and Exosome research.
Here we report an easy and quick method to analyse size and concentration of nucleic acid containing EVs by fluorescent NTA (f-NTA) after staining with DAPI.
Here we report a unique method to identify platelet-derived Exosomes from plasma samples using specific fluorescently labelled CD41 antibody and the Particle Metrix ZetaView® instrument.
Classic NTA instruments are equipped with one laser, requiring phenotyping in sequence. Multi-fluorescence detection of four biomarkers on one sample by NTA is shown for the very first time.
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ZetaView® Concentration Zeta Potential Fluorescence Size Subpopulations See what you measure Extracellular Vesicles Extracellular Vesicles Extracellular vesicles (EVs) are nanoparticles surrounded by lipid bylayers that are
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