High efficiency quantification of fluorescent labeled EVs with F-NTA
Nanoparticle Tracking Analysis (NTA) is an efficient technique for quantification of size and concentration such as virus, liposomes and EVs. Traditionally, detection of fluorescent particles (F-NTA) has been shown to be challenging due to bleaching effects known from laser induced fluorescence techniques (LIF). We present new implementations to increase efficiency and reproduciability in F-NTA.
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Along with generally accepted methods are some “tricks of the trade” such as the addition of Tween® or BSA; however, some of those additions are problematic.
Rapid determination of purity, integrity and titer
of viruses by Fluorescence Nanoparticle Tracking
Analysis (F-NTA) using the bacteriophage Phi6
Application Note Download Abstract Determination of the titer of viruses and bacteriophages is an indispensable key technology in virological research and for diagnostic purposes. Depending
The preparation of high-value biological material, such as proteins, requires a multi-step procedure of separation and purification. Each step must be optimized to reach full efficiency of yield.
Making a great exosome research tool even better, SBI has developed ExoGlow TM -NTA, a proprietary dye that enables fluorescent analysis of only the extracellular vesicles (EVs) present in a heterogeneous sample.
Reliability of concentration measurements with the Particle Metrix ZetaView® Nanoparticle Tracking Analyzer
Concentration levels of extracellular vesicles have a high clinical potential as a reference for disease.