High efficiency quantification of fluorescent labeled EVs with F-NTA

High efficiency quantification of fluorescent labeled EVs with F-NTA

Nanoparticle Tracking Analysis (NTA) is an efficient technique for quantification of size and concentration such as virus, liposomes and EVs. Traditionally, detection of fluorescent particles (F-NTA) has been shown to be challenging due to bleaching effects known from laser induced fluorescence techniques (LIF). We present new implementations to increase efficiency and reproduciability in F-NTA.

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graphic on storage conditions of extracellular vesicles (EVs)
Applications

Use of Fluorescence Nanoparticle Tracking Analysis as a tool for quality assessment of EVs

Correct and standardized storage conditions of extracellular vesicles (EVs) are essential prerequisites for the stability of clinical samples and the production of consistent EV batches for therapeutic use.
The loss of EV concentration due to improper storage conditions is associated with a reduced activity and physical integrity.
Check out our new app note on the importance of EV storage conditions and how fluorescence nanoparticle tracking analysis can be used as a quality assessment tool.

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