High efficiency quantification of fluorescent labeled EVs with F-NTA
Nanoparticle Tracking Analysis (NTA) is an efficient technique for quantification of size and concentration such as virus, liposomes and EVs. Traditionally, detection of fluorescent particles (F-NTA) has been shown to be challenging due to bleaching effects known from laser induced fluorescence techniques (LIF). We present new implementations to increase efficiency and reproduciability in F-NTA.
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Use of Fluorescence Nanoparticle Tracking Analysis as a tool for quality assessment of EVs
Correct and standardized storage conditions of extracellular vesicles (EVs) are essential prerequisites for the stability of clinical samples and the production of consistent EV batches for therapeutic use.
The loss of EV concentration due to improper storage conditions is associated with a reduced activity and physical integrity.
Check out our new app note on the importance of EV storage conditions and how fluorescence nanoparticle tracking analysis can be used as a quality assessment tool.
Detection of nucleic acid cargo in EVs by using Fluorescence Nanoparticle Tracking Analysis
The use of fluorescence nanoparticle tracking analysis (F-NTA) enables the detection of membrane-associated nucleic acids from EVs.
Analysis of 25 nm functionalized QDs using fluorescence-NTA with ZetaView® QUATT system
Application Note Download Abstract While it is uncertain how far the NTA community is able to push the limits of current technology in order to
Colocalization-NTA of multi-stained platelet derived MSC-EV preparations
After successfully showing C-NTA of double-stained MSC-EVs in our previous study (https://bit.ly/43GG0Yk) and confirming
the results with image flow cytometry, this note describes C-NTA of multi-stained MSC-EVs using a PMX-430 QUATT NTA
system with four lasers.
Specific detection of SARS-CoV-2 corona virus from cell culture supernatants by fluorescence-based Nanoparticle Tracking Analysis
In this new application note we describe a quick determination of the physical titer of coronaviruses by fluorescence-based Nanoparticle Tracking Analysis and specific spike antibody recognition.
NTA goes colocalization:
Detection and measurement of CD9 and CD41 on
double-stained human platelet derived MSC-EV
preparations
In this report, we describe the quantification of colocalization ratios on double-stained MSC-derived EVs using fluorochrome conjugated antibodies against the
cell surface antigens CD9 and CD41, which also serve as EV marker proteins.