Particle Metrix Exosomes Company History Tech Note App Note NTA See what you measure
In addition to using Nano Particle Tracking Analysis (NTA) to measurement the size distribution and concentration of EV samples, both Microfluidic Resistive Pulse Sensing (MRPS) and the Single Particle Interference Reflectance Image Sensor (SP-IRIS) methods have been widely used as an alternative means of characterizing EVs. In this note, we relay two specific cases for using the ZetaView® NTA system to achieve relative “high-throughput” analysis of many EV samples, along estimations of throughput for MRPS & SPIRIS methods for an equivalent number of samples; further, we establish realistic estimates for the high cost of ownership for operating MRPS & SP-IRIS systems as a result of the cost of consumables as well as the substantially greater amount of time spent to run the same number of samples.
Along with generally accepted methods are some “tricks of the trade” such as the addition of Tween® or BSA; however, some of those additions are problematic.
Nanoparticle Tracking Analysis (NTA) combined with surface charge measurement enables the user to gain bio-chemical information about particle surface.
Traditionally, detection of fluorescent particles (F-NTA) has been shown to be challenging due to bleaching effects known from laser induced fluorescence techniques (LIF).
Full recovery of samples measured by a nanoparticle tracking analyzer (NTA) often becomes challenging particularly if the samples are intended for further use after measurement.
From the same sample you get the parameters: number concentration, size, electrophoresis surface charge, sub-populations and fluorescence phenotyping in little time.
To anticipate the answer: With the ZetaView®.
Particle Metrix now introduces two additional types of NTA Cell Assemblies: The new types are designed for concentration, size and fluorescence detection. When working in (bio-) safety level environment, X-NTA ensures secure handling for potentially contaminating samples. In addition, zeta potential option is featured with Z-NTA Cell Assemblies.