
High Throughput ZetaView® System vs High Cost of Other Methods
Techology Note Introduction In addition to using Nano Particle Tracking Analysis (NTA) to measurement the size distribution and concentration of EV samples, both Microfluidic Resistive
0x consumables
5x faster switching
10x faster cleaning
12x fluorescence channels
∞x statistics
…and much more!
For size, concentration, zeta potential and cluster analysis of individual particles in four different fluorescence channels.
We measure your products in a measuring range of 0.8 nm to 300 μm, if possible in original concentration
Many different methods have been developed for preparation of EVs, before characterization. Along with generally accepted methods are some “tricks of the trade” such as the addition of Tween® or BSA; however, some of those additions are problematic, in that they create significant background particles.
The use of Tween® in low concentrations for EV sample preparation has been recommended by several vendors. The creation of background particles may come as a surprise to some researchers, where filtration before addition to a sample may not entirely solve the issue for a couple of reasons. First, many syringe filters shed particles of their own and require conditioning before filtration of any diluent; therefore, one must be careful not to compound the problem when taking this step. Second, as Tween® is essentially detergent, it is likely that the interaction of Tween® with the protein in EV samples will generate additional, measurable particles after a filtration step. We present here some simple tests to illustrate the problem.
Figure 1 shows Nanoparticle Tracking Analysis measurement of just PBS, a null set. Only three particles were tracked when using a typical SOP for EVs, so this PBS is clean. Figures 2, 3 and 4 show the effect of adding Tween® to PBS, even though the additive is only present in low amounts (0.1% and 0.3% concentrations). Notably, our ZetaView® system is too sensitive at a concentration of 0.5% Tween®, which creates so many surfactant nanoparticles that NTA analysis isn’t feasible.
Techology Note Introduction In addition to using Nano Particle Tracking Analysis (NTA) to measurement the size distribution and concentration of EV samples, both Microfluidic Resistive
Along with generally accepted methods are some “tricks of the trade” such as the addition of Tween® or BSA; however, some of those additions are problematic.
Platinum Presenter Talk at ISEV 2022 in Lyon Dr. Clemens Helmbrecht NTA is a multi-parameter technology Nanoparticle Tracking Analysis (NTA) is well known for measuring
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