ZetaView® Particle Tracking on Exosomes

graphic on storage conditions of extracellular vesicles (EVs)

Applications

Use of Fluorescence Nanoparticle Tracking Analysis as a tool for quality assessment of EVs

Correct and standardized storage conditions of extracellular vesicles (EVs) are essential prerequisites for the stability of clinical samples and the production of consistent EV batches for therapeutic use.
The loss of EV concentration due to improper storage conditions is associated with a reduced activity and physical integrity.
Check out our new app note on the importance of EV storage conditions and how fluorescence nanoparticle tracking analysis can be used as a quality assessment tool.

27. June 2024

EV-membrane-associated nucleic acids labeled with SYBR™ Gold.

Applications

Detection of nucleic acid cargo in EVs by using Fluorescence Nanoparticle Tracking Analysis

The use of fluorescence nanoparticle tracking analysis (F-NTA) enables the detection of membrane-associated nucleic acids from EVs.

14. May 2024

Quantum dots picture for Particle Metrix application note on analysis of 25nm functionalized QDs using F-NTA with ZetaView QUATT

Applications

Analysis of 25 nm functionalized QDs using fluorescence-NTA with ZetaView® QUATT system

Application Note Download Abstract While it is uncertain how far the NTA community is able to push the limits of current technology in order to

22. April 2024

Applications

Colocalization-NTA of multi-stained platelet derived MSC-EV preparations

After successfully showing C-NTA of double-stained MSC-EVs in our previous study (https://bit.ly/43GG0Yk) and confirming
the results with image flow cytometry, this note describes C-NTA of multi-stained MSC-EVs using a PMX-430 QUATT NTA
system with four lasers.

15. May 2023

Figure 2: SARS-CoV-2 B.1 wild type and delta variant were specifically detected by using a spike antibody conjugated with Alexa Flour™ 488.

Applications

Specific detection of SARS-CoV-2 corona virus from cell culture supernatants by fluorescence-based Nanoparticle Tracking Analysis

In this new application note we describe a quick determination of the physical titer of coronaviruses by fluorescence-based Nanoparticle Tracking Analysis and specific spike antibody recognition.

23. March 2023

Applications

NTA goes colocalization:
Detection and measurement of CD9 and CD41 on
double-stained human platelet derived MSC-EV
preparations

In this report, we describe the quantification of colocalization ratios on double-stained MSC-derived EVs using fluorochrome conjugated antibodies against the
cell surface antigens CD9 and CD41, which also serve as EV marker proteins.

1. March 2023

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ZetaView® Particle Tracking on Exosomes

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Use of Fluorescence Nanoparticle Tracking Analysis as a tool for quality assessment of EVs

Detection of nucleic acid cargo in EVs by using Fluorescence Nanoparticle Tracking Analysis

Analysis of 25 nm functionalized QDs using fluorescence-NTA with ZetaView® QUATT system

Colocalization-NTA of multi-stained platelet derived MSC-EV preparations

Specific detection of SARS-CoV-2 corona virus from cell culture supernatants by fluorescence-based Nanoparticle Tracking Analysis

NTA goes colocalization:
Detection and measurement of CD9 and CD41 on
double-stained human platelet derived MSC-EV
preparations

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