ZetaView® Particle Tracking on Exosomes

Avoiding Artifacts Figure 2: Analysis of PBS buffer containing 0.1% Tween®, using ZetaView® 488 system. A significant number of particles were measured, without EVs.

Technology

Avoiding Artifacts in EV samples:
Part 1 – the Use of Tween®

Along with generally accepted methods are some “tricks of the trade” such as the addition of Tween® or BSA; however, some of those additions are problematic.

15. September 2022

Example of an F-NTA measurement of a bacteriophage Phi6 preparation labelled with Sybr™Gold nucleic acid stain (blue curve) and Cellbrite® Fix 640 lipid layer stain (red curve) compared to scatter-based NTA (grey curve). Purity was calculated to be 82% for dsRNA and 85% for lipid layer containing phage particles.

Application

Rapid determination of purity, integrity and titer
of viruses by Fluorescence Nanoparticle Tracking
Analysis (F-NTA) using the bacteriophage Phi6

Application Note Download Abstract Determination of the titer of viruses and bacteriophages is an indispensable key technology in virological research and for diagnostic purposes. Depending

18. July 2022

Colocalization

Principles of Colocalization-NTA

Platinum Presenter Talk at ISEV 2022 in Lyon Dr. Clemens Helmbrecht NTA is a multi-parameter technology Nanoparticle Tracking Analysis (NTA) is well known for measuring

26. May 2022

Concentration, Size and Zeta Potential measured on sub-visible protein suspensions

Application

Three in One: Concentration, Size & Zeta Potential measured on sub-visible protein suspensions

The preparation of high-value biological material, such as proteins, requires a multi-step procedure of separation and purification. Each step must be optimized to reach full efficiency of yield.

24. April 2022

Application

Detect only extracellular vesicles with Nanoparticle Tracking Analysis (NTA)

Making a great exosome research tool even better, SBI has developed ExoGlow TM -NTA, a proprietary dye that enables fluorescent analysis of only the extracellular vesicles (EVs) present in a heterogeneous sample.

24. April 2022