The preparation of high-value biological material, such as proteins, requires a multi-step procedure of separation and purification. Each step must be optimized to reach full efficiency of yield.
Making a great exosome research tool even better, SBI has developed ExoGlow TM -NTA, a proprietary dye that enables fluorescent analysis of only the extracellular vesicles (EVs) present in a heterogeneous sample.
Reliability of concentration measurements with the Particle Metrix ZetaView® Nanoparticle Tracking Analyzer
Concentration levels of extracellular vesicles have a high clinical potential as a reference for disease.
F-NTA of fluorescent labelled bionanoparticles is an efficient technique for quantification of size and total concentration.
Shown with four-fold labeled EVs: Multi-wavelength NTA reduces total measurement time, minimizes the amount of sample required, and improves experimental reproducibility.
The particular area of expertise focuses on investigating the roles of extracellular vesicles (EVs) in cancer and cellular stress responses.
From the same sample you get the parameters: number concentration, size, electrophoresis surface charge, sub-populations and fluorescence phenotyping in little time.
Nanoparticle Tracking Analysis (NTA) combined with surface charge measurement enables the user to gain bio-chemical information about particle surface.
Traditionally, detection of fluorescent particles (F-NTA) has been shown to be challenging due to bleaching effects known from laser induced fluorescence techniques (LIF).
Full recovery of samples measured by a nanoparticle tracking analyzer (NTA) often becomes challenging particularly if the samples are intended for further use after measurement.