Use of Fluorescence Nanoparticle Tracking Analysis as a tool for quality assessment of EVs
![graphic on storage conditions of extracellular vesicles (EVs)](https://particle-metrix.com/wp-content/uploads/2024/06/Thumbnail-Particle-Metrix-Appnote-EV-storage-conditions-1024x499.png)
Correct and standardized storage conditions of extracellular vesicles (EVs) are essential prerequisites for the stability of clinical samples and the production of consistent EV batches for therapeutic use.
The loss of EV concentration due to improper storage conditions is associated with a reduced activity and physical integrity.
Check out our new app note on the importance of EV storage conditions and how fluorescence nanoparticle tracking analysis can be used as a quality assessment tool.
Detection of nucleic acid cargo in EVs by using Fluorescence Nanoparticle Tracking Analysis
![EV-membrane-associated nucleic acids labeled with SYBR™ Gold.](https://particle-metrix.com/wp-content/uploads/2024/05/EV-membrane-associated-nucleic-acids_300px.png)
The use of fluorescence nanoparticle tracking analysis (F-NTA) enables the detection of membrane-associated nucleic acids from EVs.
Analysis of 25 nm functionalized QDs using fluorescence-NTA with ZetaView® QUATT system
![Quantum dots picture for Particle Metrix application note on analysis of 25nm functionalized QDs using F-NTA with ZetaView QUATT](https://particle-metrix.com/wp-content/uploads/2024/04/QDs-for-Particle-Metrix-Application-Note_frame-600.jpg)
Application Note Download Abstract While it is uncertain how far the NTA community is able to push the limits of current technology in order to detect the smallest possible nanoparticles, the results of analyzing 25 nm quantum dots functionalized with streptavidin (S-QDs) show that the ZetaView® QUATT system from Particle Metrix is the leader in […]
Colocalization-NTA of multi-stained platelet derived MSC-EV preparations
![](https://particle-metrix.com/wp-content/uploads/2023/05/Particle-Metrix-AppNote-Colocalization-NTA-Multistaining-1024x424.png)
After successfully showing C-NTA of double-stained MSC-EVs in our previous study (https://bit.ly/43GG0Yk) and confirming
the results with image flow cytometry, this note describes C-NTA of multi-stained MSC-EVs using a PMX-430 QUATT NTA
system with four lasers.
Specific detection of SARS-CoV-2 corona virus from cell culture supernatants by fluorescence-based Nanoparticle Tracking Analysis
![Figure 2: SARS-CoV-2 B.1 wild type and delta variant were specifically detected by using a spike antibody conjugated with Alexa Flour™ 488.](https://particle-metrix.com/wp-content/uploads/2023/03/2022_sarscov2_fig_02.jpg)
In this new application note we describe a quick determination of the physical titer of coronaviruses by fluorescence-based Nanoparticle Tracking Analysis and specific spike antibody recognition.
NTA goes colocalization:
Detection and measurement of CD9 and CD41 on
double-stained human platelet derived MSC-EV
preparations
![](https://particle-metrix.com/wp-content/uploads/2023/02/Thumbnail_Coloco_CD41_CD9-1024x679.jpg)
In this report, we describe the quantification of colocalization ratios on double-stained MSC-derived EVs using fluorochrome conjugated antibodies against the
cell surface antigens CD9 and CD41, which also serve as EV marker proteins.
High Throughput ZetaView® System vs High Cost of Other Methods
![](https://particle-metrix.com/wp-content/uploads/2022/05/X30_V2_Mono_Twin_Quatt_cmyk_400px.jpg)
In addition to using Nano Particle Tracking Analysis (NTA) to measurement the size distribution and concentration of EV samples, both Microfluidic Resistive Pulse Sensing (MRPS) and the Single Particle Interference Reflectance Image Sensor (SP-IRIS) methods have been widely used as an alternative means of characterizing EVs. In this note, we relay two specific cases for using the ZetaView® NTA system to achieve relative “high-throughput” analysis of many EV samples, along estimations of throughput for MRPS & SPIRIS methods for an equivalent number of samples; further, we establish realistic estimates for the high cost of ownership for operating MRPS & SP-IRIS systems as a result of the cost of consumables as well as the substantially greater amount of time spent to run the same number of samples.
Rapid determination of purity, integrity and titer
of viruses by Fluorescence Nanoparticle Tracking
Analysis (F-NTA) using the bacteriophage Phi6
![Example of an F-NTA measurement of a bacteriophage Phi6 preparation labelled with Sybr™Gold nucleic acid stain (blue curve) and Cellbrite® Fix 640 lipid layer stain (red curve) compared to scatter-based NTA (grey curve). Purity was calculated to be 82% for dsRNA and 85% for lipid layer containing phage particles.](https://particle-metrix.com/wp-content/uploads/2022/07/phi6_grafik_web_02.png)
Determination of the titer of viruses and bacteriophages is an indispensable key technology in virological research and for diagnostic purposes. Depending on the method used, the measurements are either qualitative or quantitative, very time-consuming, and do not provide information about integrity or aggregation behaviour of the virus particles. Nanoparticle Tracking Analysis with the Particle Metrix ZetaView® instrument allows the user to perform a rapid concentration determination of virus particles. Using the bacteriophage Phi6 as an example, we show how titer, purity and integrity of phage particles can be measured quickly and reliably using the fluorescence detection capability of the Particle Metrix ZetaView® instrument.
Three in One: Concentration, Size & Zeta Potential measured on sub-visible protein suspensions
![Concentration, Size and Zeta Potential measured on sub-visible protein suspensions](https://particle-metrix.com/wp-content/uploads/2022/04/Concentration-Size-and-Zeta-Potential-measured-on-sub-visible-protein-suspensions.png)
The preparation of high-value biological material, such as proteins, requires a multi-step procedure of separation and purification. Each step must be optimized to reach full efficiency of yield.
Detect only extracellular vesicles with Nanoparticle Tracking Analysis (NTA)
![EV Isolation Yields confirmed via F-NTA](https://particle-metrix.com/wp-content/uploads/2022/04/EV-Isolation-Yields-confirmed-via-F-NTA.png)
Making a great exosome research tool even better, SBI has developed ExoGlow TM -NTA, a proprietary dye that enables fluorescent analysis of only the extracellular vesicles (EVs) present in a heterogeneous sample.