ZetaView® x20 Series

Nanoparticle Tracking Video Microscope

For size, concentration, zeta potential and cluster analysis of individual particles in four different fluorescence channels

ZetaView® QUATT

Nanoparticle Tracking Video Microscope PMX-420

For size, concentration, zeta potential and cluster analysis of individual particles in four different fluorescence channels

ZetaView® x20 Series

Perfect for biological nanoparticles

Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are in the growing interest of the biosciences and nanomedicine. With the NTA technology, particles are individually measurable in a physiological buffer and you observe what you measure in a video. A new step forward in convenience and speed for fluorescence studies of EV-antibody conjugates has been achieved with the ZetaView® QUATT Lasers and fluorescence filters are pre-selectable via the software. This procedure saves time and sample.

Typical application

Determining the absolute concentration is one of the strengths of the NTA technique. In scatter mode, the concentration of all particles is measured. Changing subsequently to the fluorescence mode, marked biologic nanoparticles are tracked. By using four lasers within a SOP assisted sequence, it is possible to differentiate between four different fluorescence dyes in the same sample.

Phenotyping of tetraspanins for instance, on vesicles and virus is facilitated, positive and isotype control as well.

To prepare instrument settings, standard EVs may be used.

Your benefits at a glance

4 fluorescence channels per sample

4 fluorescence channels per sample

By using four lasers within a SOP assisted sequence, it is possible to differentiate between four different fluorescence dyes in the same sample.

Change channels with a mouse click

Change channels with a mouse click

Laser and fluorescence channels can be switched with a mouse click.

Upgradable from PMX-120 Basic

Upgradable from PMX-120 BASIC to PMX-220 TWIN or PMX-420 QUATT

Upgradable from PMX-120 Basic

Analysis of over 2,000 par­ticles in as little as 60 seconds.

Scanning NTA

Scanning NTA

Automated measurements taken at 11 distinct positions in the sample cell offer a more representative analysis than single-point measurements. Outlier control selects flawless videos. Autoalignment and Autofocus keep the video quality constant.

Auto-alignment & auto-focus

Auto-alignment & auto-focus

The optical setup is automatically optimized by the software, saving the user time preparing the instrument for use and removing subjective user input bias.

Fast measurements

Fast measurements

Analysis of over 2,000 par­ticles in as little as 60 seconds.

Quick and simple cleaning

Quick and simple cleaning

Cleaning between samples requires only a quick flush.

Intuitive software

Intuitive software

Traffic-light system gives an instant indication of whether the sample is at a sui­table concentration for measurement and indicates the dilution factor of the sample.

Compact all-in-one design

Compact all-in-one design

Measure size, con­centration, zeta potential and fluorescence with one instrument. Compatible with the laboratory environment; small footprint and data files.

Fluorescence analysis

Fluorescence analysis

Fluorescence NTA mea­surements allow interrogation of subpopulations in the sample. A sensitive CMOS camera, selective filters, and low bleaching performance yield high fluorescence sensitivity.

No high cost consumables

No high cost consumables

Just syringes and reference beads for scattered light and fluorescence measurements, if applicable. The life time of the quartz cell is several years. It is easy to clean with pipe cleaners.

No calibration

No calibration

Size measurements do not need to be calibrated as the method is absolute.

For more information on the ZetaView® or to discuss your requirements, please contact us.

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