ZetaView® TWIN

Nanoparticle Tracking Video Microscope PMX-220

For size, concentration, zeta potential and cluster analysis of individual particles in two different fluorescence channels

ZetaView® TWIN

Nanoparticle Tracking Video Microscope PMX-220

For size, concentration, zeta potential and cluster analysis of individual particles in two different fluorescence channels

ZetaView® TWIN

Perfect for biological nanoparticles

Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or virus-like particles are in the growing interest of the biosciences and nanomedicine. With the NTA technology, particles are individually measurable in a physiological buffer and you observe what you measure in a video. A new step forward in convenience and speed for fluorescence studies of EV-antibody conjugates has been achieved with the ZetaView® TWIN. Lasers and fluorescence filters are pre-selectable via the software. This procedure saves time and sample.

Typical application

Determining the absolute concentration is one of the strengths of the NTA technique. In scatter mode, the concentration of all particles is measured. Changing subsequently to the fluorescence mode, marked biologic nanoparticles are tracked. By using two lasers within a SOP assisted sequence, it is possible to differentiate between two different fluorescence dyes in the same sample.

Phenotyping of tetraspanins for instance, on vesicles and virus is facilitated, positive and isotype control as well.

To prepare instrument settings, standard EVs may be used.

Your benefits at a glance

2 fluorescence channels per sample

By using two lasers within a SOP assisted sequence, it is possible to differentiate between two different fluorescence dyes in the same sample.

Change channels with a mouse click

Laser and fluorescence channels can be switched with a mouse click.

Scanning NTA

Automated measurements taken at 11 distinct positions in the sample cell offer a more representative analysis than single-point measurements. Outlier control selects flawless videos. Autoalignment and Autofocus keep the video quality constant.

Auto-alignment & auto-focus

The optical setup is automatically optimized by the software, saving the user time preparing the instrument for use and removing subjective user input bias.

Fast measurements

Analysis of over 2,000 particles in as little as 60 seconds.

Quick and simple cleaning

Cleaning between samples requires only a quick flush.

Intuitive software

Traffic-light system gives an instant indication of whether the sample is at a suitable concentration for measurement and indicates the dilution factor of the sample.

Compact all-in-one design

Measure size, concentration, zeta potential and fluorescence with one instrument. Compatible with the laboratory environment; small footprint and data files.

Fluorescence analysis

Fluorescence NTA measurements allow interrogation of subpopulations in the sample. A sensitive CMOS camera, selective filters, and low bleaching performance yield high fluorescence sensitivity.

No high cost consumables

Just syringes and reference beads for scattered light and fluorescence measurements, if applicable. The life time of the quartz cell is several years. It is easy to clean with pipe cleaners.

No calibration

Size measurements do not need to be calibrated as the method is absolute.

Technology

Nanoparticle Tracking Analysis

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ZetaView® TWIN

Nanoparticle Tracking Video Microscope PMX-220

ZetaView® TWIN

Nanoparticle Tracking Video Microscope PMX-220

ZetaView® TWIN

Perfect for biological nanoparticles

Typical application