ZetaView® Subpopulations Size Fluorescence Zeta Potential Concentration Colocalization Next Generation NTA
Applications
EXTRACELLULAR VESICLES
Research on Exacellular Vesicles is thriving. Nanoparticle Tracking Analysis plays an important role in this development due to in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics
Viruses
Nanoparticle Tracking Analysis can be used to quantify total concentration of virions, analyse the amount of successfully loaded virions as well as virus aggregatiion.
Biological nanoparticles, such as extracellular vesicles (EVs), exosomes, lipids, viruses or proteins can be found in almost all fluids of living tissue. The Particle Metrix ZetaView® Nanoparticle Tracking Analysis technology is capable to characterize them reliably in water or physiological buffers. You can simultaneously get information about particle size, particle concentration and surface charge with just one single measurement of a particle sample. Similarly, cluster analysis based on similar properties is possible.
For more information on the ZetaView® or to discuss your requirements, please contact us.
Upcoming Events
IGLD 2023
23 March 2023 – 25 March 2023
Frankfurt, Germany
32nd Annual Meeting of the Society for Virology
28 March 2023 – 31 March 2023
Ulm, Germany
2023 Asia Pacific Societies for Extracellular Vesicles Conference
31 March 2023 – 2 April 2023
Taipei, Taiwan
Get the news
NTA goes colocalization:
Detection and measurement of CD9 and CD41 on
double-stained human platelet derived MSC-EV
preparations
In this report, we describe the quantification of colocalization ratios on double-stained MSC-derived EVs using fluorochrome conjugated antibodies against the
cell surface antigens CD9 and CD41, which also serve as EV marker proteins.
High Throughput ZetaView® System vs High Cost of Other Methods
In addition to using Nano Particle Tracking Analysis (NTA) to measurement the size distribution and concentration of EV samples, both Microfluidic Resistive Pulse Sensing (MRPS) and the Single Particle Interference Reflectance Image Sensor (SP-IRIS) methods have been widely used as an alternative means of characterizing EVs. In this note, we relay two specific cases for using the ZetaView® NTA system to achieve relative “high-throughput” analysis of many EV samples, along estimations of throughput for MRPS & SPIRIS methods for an equivalent number of samples; further, we establish realistic estimates for the high cost of ownership for operating MRPS & SP-IRIS systems as a result of the cost of consumables as well as the substantially greater amount of time spent to run the same number of samples.
Avoiding Artifacts in EV samples:
Part 1 – the Use of Tween®
Along with generally accepted methods are some “tricks of the trade” such as the addition of Tween® or BSA; however, some of those additions are problematic.