Fluorescence Detection
Fluorescence Detection is an indispensable cornerstone of modern nanoparticle analysis, enabling researchers to identify and characterize specific particle subpopulations within their samples. With Particle Metrix’s ZetaView® systems, fluorescence detection is seamlessly integrated into multi-parameter analysis, offering unmatched versatility and accuracy across diverse applications.
What is Fluorescence Detection?
Fluorescence Detection is a technique that uses the emission of light by fluorescently labeled particles when exposed to specific wavelengths. This enables the identification and analysis of targeted particles within a complex sample. By tagging particles with fluorescent dyes, researchers can study their properties, interactions, and behaviors with high specificity.
Fluorescence detection is essential for understanding the composition of biological samples, tracking labeled subpopulations, and distinguishing particles based on biological or chemical markers. This capability is invaluable for applications in life sciences, pharmaceuticals, and materials research, where identifying and characterizing distinct populations is critical. Key Attributes of Fluorescence Detection:
Specific Identification: Fluorescence enables detection of specific particle types within heterogeneous samples.
Multi-Channel Analysis: Analyze multiple fluorescence signals simultaneously to characterize complex polydisperse samples.
Enhanced Characterization: Combine fluorescence data with size and concentration measurements for comprehensive insights.
Why is Fluorescence Detection important?
The ability to detect fluorescence is vital for advancing research and product development in fields requiring precise particle characterization. Fluorescence Detection allows researchers toanalyze biological or synthetic samples with unmatched specificity. For instance:
In pharmaceuticals, fluorescence detection is used to verify the labeling of drug carriers and study their interactions with biological targets.
In biotechnology, researchers rely on fluorescence to identify Extracellular Vesicles (EVs) or other biological nanoparticles marked with fluorescent dyes.
In life-sciences, fluorescent labeling is key to identify extracellular vesicles or other biological particles in patient samples.
In materials science, fluorescence helps to characterize nanoparticles used in imaging, diagnostics, and advanced material designs.
How is Fluorescence detected?
Fluorescence detection is performed using advanced systems like the ZetaView® NTA, which integrates fluorescence capabilities into multi-parameter analysis. Fluorescence detection with the ZetaView®:
- Particles are tagged with fluorescent markers that emit light when exposed to specific laser wavelengths.
- The ZetaView® system uses up to four lasers (e.g., 405 nm, 488 nm, 532 nm, 660 nm) to excite the fluorescent tags.
- High-sensitivity CMOS cameras capture the emitted fluorescence, enabling the identification and characterization of labeled subpopulations.
This process ensures accurate detection and comprehensive analysis of fluorescently labeled particles within complex samples.
Advanced Fluorescence Detection with ZetaView®
The ZetaView® system offers precise and automated fluorescence detection, providing detailed insights into labeled subpopulations while simultaneously analyzing size and concentration.
Key advantages of ZetaView® for fluorescence detection include:
Multi-Channel Fluorescence
Analyze up to four fluorescence signals simultaneously without manual adjustments.
High Sensitivity
Detect even low concentrations of fluorescently labeled particles.
Advanced Colocalization
Identify and quantify particles co-labeled with multiple fluorescent markers for deeper insights into particle interactions.
Streamlined Workflows
Automated sample handling ensures efficient and reproducible measurements.
Versatile Applications
Tailored solutions for biological, synthetic, and environmental samples.
Applications of Fluorescence Detection
Fluorescence Detection is widely used across various industries, supporting research, quality control, and innovation:
Pharmaceuticals: Analyze labeled drug carriers to study biodistribution, target interactions, and therapeutic efficacy.
Biotechnology and Life Sciences: Identify fluorescently tagged subpopulations of EVs, and other biologically relevant particles.
Materials Science: Characterize fluorescent nanoparticles used in sensors, imaging agents, and smart materials.
Environmental Science: Track fluorescently labeled pollutants or nanoparticles in ecosystems to assess their impact.
Let’s talk about your Fluorescence Detection needs
If you’re ready to explore how fluorescence detection can enhance your research or product development, connect with our team of experts. We offer personalized consultations, system demonstrations, and technical support to help you achieve your goals.
Frequently asked questions about Fluorescence Detection
What types of samples can be analyzed using fluorescence detection?
Fluorescence detection is suitable for nanoparticles, extracellular vesicles, and synthetic materials tagged with fluorescent markers.
What are the advantages of using Particle Metrix for fluorescence detection?
Our technology offers multi-channel fluorescence, high sensitivity, and size and concentration measurements in fluorescence as well as in scatter mode, providing a comprehensive understanding of your samples.
How do I ensure accurate fluorescence detection?
Use high-quality fluorescent markers and our well-established staining protocols, prepare samples consistently, and choose optimal instrument settings.
Can the ZetaView® system analyze multi-labeled particles?
Yes, the ZetaView® system supports advanced multi-fluorescence capabilities, enabling the analysis of particles labeled with up to 4 fluorescent markers.
