ZetaView® Subpopulations Size Fluorescence Zeta Potential Concentration Colocalization Next Generation NTA

Applications

3d render of cells secreting exosomes

EXTRACELLULAR VESICLES

Research on Exacellular Vesicles is thriving. Nanoparticle Tracking Analysis plays an important role in this development due to in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics

particlemetrix-virus

Viruses

Nanoparticle Tracking Analysis can be used to quantify total concentration of virions, analyse the amount of successfully loaded virions as well as virus aggregatiion.

Biological nanoparticles, such as extracellular vesicles (EVs), exosomes, lipids, viruses or proteins can be found in almost all fluids of living tissue. The Particle Metrix ZetaView® Nanoparticle Tracking Analysis technology is capable to characterize them reliably in water or physiological buffers. You can simultaneously get information about particle size, particle concentration and surface charge with just one single measurement of a particle sample. Similarly, cluster analysis based on similar properties is possible.

The x30 Series at a Glance

For more information on the ZetaView® or to discuss your requirements, please contact us.

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Technology

High Throughput ZetaView® System vs High Cost of Other Methods

In addition to using Nano Particle Tracking Analysis (NTA) to measurement the size distribution and concentration of EV samples, both Microfluidic Resistive Pulse Sensing (MRPS) and the Single Particle Interference Reflectance Image Sensor (SP-IRIS) methods have been widely used as an alternative means of characterizing EVs. In this note, we relay two specific cases for using the ZetaView® NTA system to achieve relative “high-throughput” analysis of many EV samples, along estimations of throughput for MRPS & SPIRIS methods for an equivalent number of samples; further, we establish realistic estimates for the high cost of ownership for operating MRPS & SP-IRIS systems as a result of the cost of consumables as well as the substantially greater amount of time spent to run the same number of samples.

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